Comparative matching of the 2D PAGE images, performed by the PDQUEST software (v.8.0.1, Bio-Rad) using several reference proteins (Figs. 1B and D, respectively, red arrows), showed an exact overlapping of the position of the protein spots detected by the BLCAP antibody in breast tumor samples and BLCAP overexpressing COS-1 cells. This evidence concerns the gene BLCAP and breast neoplasm.