On RT-PCR assay, the three HCC cell lines all exhibited an aberrant overexpression of COMMD7 mRNA relative to β-actin as compared to normal hepatocyte cell L02, whereas HepG2 cells expressed a significantly higher level of COMMD7 mRNA than the other two HCC cell lines, namely, Huh7 and Hep3B (HepG2 vs. Huh7 vs. Hep3B vs. L02, 1.613±0.238 vs 0.874±0.106 vs.0.793±0.116 vs. 0.529±0.065, P<0.01; Figure 1A). This evidence concerns the gene ACTB and hepatocellular carcinoma.