Specifically, DDIT3, HOXD10, PDE1C and PLS3 were upregulated in GNS cells and expressed at higher levels in glioblastomas with poor prognosis, while PTEN and TUSC3 were downregulated in GNS cells and expressed at lower levels in gliomas with poor prognosis. The gene discussed is PLS3; the disease is glioblastoma.