In the present study, Ezrin protein was found to locate in the cytoplasm and/or membrane in the migrating gastric cancer cells in vitro, and it mainly concentrated at the protrusion site of MKN-1 gastric cancer cells; however, Ezrin protein located only in the cytoplasm in non-migrating cells in vitro by immunofluorescence staining (Figure1). This evidence concerns the gene EZR and gastric cancer.