The significant differential methylation status of HIST1H3C and ACSS3 between MYCN single copy and MNA samples was confirmed in the NB cell lines (HIST1H3C, methylated in 15/21 (71%) MNA cell lines and in 2/10 (20%) MYCN single copy cell lines, P = 0.018; ACSS3, methylated in 20/21 (95%) MNA cell lines and in 3/10 (30%) MYCN single copy cell lines, P < 0.001). Here, ACSS3 is linked to neuroblastoma.