The cell viability was determined by MTT, and the effect of mesothelin shRNA on the growth of cancer cells is shown in Figure4A. We found that down-regulation of mesothelin expression significantly caused cell growth inhibition in the ASPC-1 and CaPan-2 pancreatic cancer cell lines (Figure4A, P<0.05,respectively). The gene discussed is MSLN; the disease is pancreatic neoplasm.