Biologic inhibition assays in hepatoma-derived cell cultures using soluble RGM.Fc fusion proteins to inhibit BMP ligand-mediated hepcidin promoter luciferase activity have previously demonstrated that HJV.Fc inhibited BMP4, BMP2, BMP6 and BMP5 most robustly with lesser inhibition of BMP7 and no inhibition of BMP9 [19], while RGMB.Fc inhibited BMP4 and BMP2 most robustly, with lesser inhibition of BMP6, BMP5, and BMP7 and no inhibition of BMP9 [18]. This evidence concerns the gene BMP2 and hepatocellular carcinoma.