In order to examine whether the CA/E3/09 infection generates responses to the predicted CD8 epitopes, we synthesized the 28 peptides listed in Table 1 and 2, which included a total of 12 peptides identical between X31 and CA/E3/09 virus, and used IFN-γ ELISPOT assay to detect T cell responses. This evidence concerns the gene IFNG and infection.