Tau and phospho-tau concentrations in the formic acid extracts were significantly higher in females as evidenced using two-way ANOVAs (Fig. 3 A, B, C, D) Correlative analyses also suggested that the behavioural expression of progressing tau pathology in 3xTg-AD mice differed between males and females (Fig. 3 E, F, G, H). Here, MAPT is linked to Alzheimer disease.