The western analysis results revealed the ratio of LC3-II/LC3-I and the expression of Beclin 1 increased as early as 1 h after ischemia, augmented significantly at 6 h, peaked at 24 h and lasted for 48 h postischemia (Figure 1A–B; *p<0.05 vs. the Sham group; #p<0.05 vs. I/R-24 h group). This evidence concerns the gene BECN1 and ischemia.