To determine the expression levels of beclin-1 transcripts and protein, semi-quantitative reverse transcription-PCR analysis and western blotting analysis were conducted separately in normal human gastric epithelial cells (GES-1) and six gastric cancer cell lines (HGC-27,MKN803,MGC-803,SGC-7901, MKN-28,BGC-823). This evidence concerns the gene BECN1 and gastric cancer.