In the current study, publicly available algorithms (TargetScan, Pictar, miRANDA) indicated that miR-370 may directly target the 3`-UTR of FOXO1. We demonstrated that miR-370 promoted prostate cancer cell proliferation by directly targeting the 3′-UTR of FOXO1 mRNA, which subsequently reduced expression of the cyclin-dependent kinase (CDK) inhibitors, p27Kip1 and p21Cip1, and upregulated the cell-cycle regulator cyclin D1. This evidence concerns the gene FOXO1 and prostate cancer.