INS and obesity disorder: Our current research goal was to investigate the potential role of acylated ghrelin on the development of human obesity by examining: 1) the response of fasting serum acylated ghrelin concentrations in normal weight, overweight, and obese males to short-term overfeeding; 2) fasting serum acylated ghrelin concentrations in each adiposity group before and after overfeeding; and 3) the relationships of fasting serum acylated ghrelin concentrations with fasting glucose and insulin concentrations, blood lipids, and insulin resistance state, before and after overfeeding.