As hydrocephalus development in JAM-C−/− mice C57BL/6 mice strongly suggested the expression of JAM-C in the CNS, we performed double-immunofluorescence stainings for JAM-C and PECAM-1 on brain cryosections from wildtype and JAM-C−/− C57BL/6 mice. Here, JAM3 is linked to Hydrocephalus.