JAM3 and Hydrocephalus: As expected, in wildtype and non-hydrocephalic JAM-C−/− C57BL/6 mice DiI was detectable in and around the lateral, 3rd and 4th ventricles, while in JAM-C−/− C57BL/6 mice with symptomatic hydrocephalus the dye was prevented to reach the 4th or even the 3rd ventricle.