In the present study, we conducted a detailed mechanistic study to evaluate the efficacy of inhibition of β-catenin/Tcf-4 signaling in human CRC using the human CRC SW480 cell line, which harbors mutant APC and wild-type β-catenin, and the HCT116 cell line, which harbors mutant β-catenin and wild-type APC. This evidence concerns the gene TCF4 and colorectal carcinoma.