To investigate the impact of macrophage c-Myc deletion on the pro-tumoral activity of TAMs, we sorted B16 melanoma tumor cell suspensions and analyzed by qPCR the expression of three factors required for tissue remodeling and angiogenesis in cancer: hypoxia-inducible factor 1α (HIF1α), vascular endothelial growth factor (VEGFA) and metalloproteinase-9 (MMP9). This evidence concerns the gene MMP9 and neoplasm.