The mRNA and protein expression profiles of SphK1 and SphK2 were examined in one control (Met5A, non-malignant transformed mesothelioma cells) and three mesothelioma cell lines using real time RT-PCR and immunoblotting with anti-SphK1 and anti-SphK2 polyclonal antibodies. This evidence concerns the gene SPHK1 and mesothelioma.