Modulation of SOCS3 expression in HNSCC cell lines was maximum 48 h after transfection in both cell lines and for gain of function (overexpression by CMV-driven plasmid - Figure 3, A and B) and loss of function (siRNA, Figure 3, E), so this period was used for all the subsequent experiments involving gain and loss of function. The gene discussed is SOCS3; the disease is head and neck squamous cell carcinoma.