Nosignificant differences in the expression of TLR2 and TLR4 were observed in the surface ofIECs, between the whole group of IBS patients and controls (data not shown); however, thefluorescence intensity of the TLRs varied markedly between the different IBS subtypes.TLR4 surface level was significantly increased in IBS-M patients compared to controls,which is in line with the real-time PCR results (figure 4B). This evidence concerns the gene TLR4 and irritable bowel syndrome.