Finally, in order to firmly demonstrate that the modulation of the levels of LEDGF, Nup153 and ADAM10 by miR-155 mediates the reduced susceptibility to infection in macrophages, through an accumulation of late RT products and absence or large reduction in integrated pro-virus, we performed a silencing experiment in which the expression of the above mentioned HDFs was reduced individually, or in various combinations, in a TLR- and miR-155-independent context, using Silencer Select siRNAs (Ambion). This evidence concerns the gene PSIP1 and infection.