Moreover, when we analyzed the prognostic impact of c-Myc and SIRT1 for HCC patients with the combination of these two molecules, patients expressing both c-Myc and SIRT1 displayed shorter OS and DFS compared with the patients that did not express both c-Myc and SIRT1 (mean overall survival time; 48.45±7.28, 115.08±7.66 months, respectively) (Figure S4). This evidence concerns the gene SIRT1 and hepatocellular carcinoma.