M2 cells are not inherently motile; thus to study the effects of DIP upon morphological transitions in motile cancer cells, a panel of human breast cancer cell lines was screened for DIP and mDia2 expression, including epithelial- (MCF10A, MCF7), mesenchymal- (HS578T, MDA-MB-231) or amoeboid- (TMX2-28, MDA-MB-435S) shaped cells. This evidence concerns the gene DIAPH3 and cancer.