In our study, we found that hypothermia induced by 5′-AMP has protective effects on endotoxemia, as demonstrated by the following criteria: (1) a significant reduction of IL-1β (P < 0.05), IL-6 (P < 0.05), TNF-α (P < 0.05) and an increase in IL-10 (P < 0.05) in the plasma and BALF levels in rats that were pre- and posttreated with 5′-AMP, when compared with rats injected with LPS only; (2) a significant inhibition of acute lung injury induced by LPS. This evidence concerns the gene IL6 and serum lipopolysaccharide activity.