In order to elucidate if this effect could be extended to other cancer cells, effect of 5′-DFUR and gemcitabine treatment on AQP3 expression and cell volume were tested in the colon carcinoma cell line HT29, the pancreatic cancer cell line NP-29 and the ER/PR negative breast cancer derived MDA-MB-468. The gene discussed is ESR1; the disease is breast cancer.