Here we found that serum mesothelin provided the highest area under the ROC curve (0.94, 95% CI 0.87-1.00) followed by pleural fluid mesothelin (0.89, 95% 0.77-1.00) and pleural effusion DNA integrity index (0.82, 95% CI 0.68-0.97). The gene discussed is MSLN; the disease is Pleural effusion.