In the present study, 23 of 43 (53.5%) methylated gastric carcinoma tissues lost RB protein expression, which was higher than that in unmethylated gastric carcinoma tissues (32.0%, 8/25), but RB promoter methylation was not reversely correlated with RB protein expression (P = 0.086). The gene discussed is RB1; the disease is gastric carcinoma.