DCN and neoplasm: When methods like Northern (Tralhão et al. 2003) or western blot analyses (Leygue et al. 2000; Nash et al. 2002; Troup et al. 2003) and more modern methods such as RT-PCR (Eshchenko et al. 2007) are used to evaluate the expression of molecules, e.g., decorin, they are usually performed with pooled samples consisting of the sectioned tumor which also contain its surrounding original stroma.