The tags were introduced into the pET expression vector[17] as in frame fusions with two single-chain antibody fragments: (1) as proof of concept, the anti-CD30 antibody Ki-4(scFv)[18], allowing functional analysis in the CD30-positive L540cy human Hodgkin’s lymphoma cell line[18]; and (2) for additional measurements the anti-MucI antibody M12(scFv)[19] present on the human mamma carcinoma cell line MCF7. Here, TNFRSF8 is linked to Hodgkins lymphoma.