PHF1 and tauopathy: However, it is important to emphasize that Atg7-deficiency does not appear to induce a full tauopathy pathology, as not all phospho-tau epitopes are observed (e.g., PHF1 antibody is negative, Figure4e), and amyloid staining with Thioflavin S, as well as electron microscopic analysis, do not support the presence of mature NFTs.