This inference is supported by the observation that in colon cancer SW620 cells, the level of methylated promoter of miR-34a was decreased in response to CDF treatment, and that this reduction was accompanied by concomitant reduction in Notch-1 expression, one of the targets of miR-34a which is consistent with our previous finding on Notch-1 expression after CDF treatment and after miR34a induction mediated by other demethylating agents [23,29]. This evidence concerns the gene NOTCH1 and malignant colon neoplasm.