To further investigate the involvement of PI3K/Akt/mTOR signaling in the AST-induced anti-angiogenic effects against colon cancer, HCT 116 cells were treated with PI3K inhibitor LY294002 (50 μM), Akt inhibitor (10 μM) or mTOR inhibitor rapamycin (50 nM), respectively. Here, AKT1 is linked to malignant colon neoplasm.