Real-time RT-PCR analysis for Hes1 gene in striatum after MCAO (Figure2A) showed that in EA group, Hes1 mRNA rapidly increased to the maximum at 2 h after reperfusion (P < 0.05 vs. before I/R), and then gradually returned to the its pre-brain ischemia level. The gene discussed is HES1; the disease is brain ischemia.