The dynamic of Hspb1 gene expression was characterized by a significant enrichment found at pre-malignant and non-metastatic stages (4C melanocyte lineage and 4C11− melanoma cell line, respectively) in relation to melan-a melanocytes, followed by a progressive loss in Hspb1 expression in 4C11+ metastatic melanoma cell line when compared with 4C pre-malignant melanocyte and 4C11− non-metastatic melanoma cell. The gene discussed is HSPB1; the disease is melanoma.