To study functional importance of uPAR and MMP-9 in medulloblastoma progression, Daoy and D283 cells were transfected with shRNA plasmids targeted against uPAR (pU), MMP-9 (pM), either alone or simultaneously (pUM) in combination with radiation (IR) treatment and compared with cells transfected with either transfection reagent (control/mock) or pSV (vector with scrambled non-specific sequence). The gene discussed is PLAUR; the disease is medulloblastoma.