SPI1 and plasma cell myeloma: However, after treatment with the DNA demethylating agent decitabine at 1 μM for 3 days, leading to mRNA reactivation in KMS-12-PE myeloma cells, similar DC-PCR results were obtained as in cells expressing CDKN2A (Normal Human Dermal Fibroblasts, NHDF) or PU.1 (RPMI 8226 myeloma cells) at baseline (Figures 1B and 2B).