The protein expression levels of NF-κB, p-NF-κB, IκBα, p-IκBα, IKKα, and p-IKKα were tested in Nix knockdown (Nix-kn) and control (Nix-wt) U251 glioblastoma cell lines under conditions of normoxia and hypoxia. Here, NFKBIA is linked to glioblastoma.