ANXA5 and hepatocellular carcinoma: To confirm that the effects of Tg737 overexpression on the facilitation of HCC cell adhesion and on the attenuation of invasion and migration under hypoxic conditions were not due to decreased cell viability resulting from transfection with pcDNA3.1-Tg737, we assessed the effect of pcDNA3.1-Tg737 transfection on cell viability using Annexin V assays.