To determine the role of coronin 3 expression in the malignant behavior of gastric cancer cells, a lentivirus containing an shRNA construct (shRNA-LV) was constructed to down-regulate coronin 3 expression in MKN45 cells, and pcDNA3.1-Coronin 3 was transfected to MKN28-NM cells to up-regulate coronin 3 expression. This evidence concerns the gene CORO1C and gastric cancer.