The present study used cultured human prostate cancer cells (PC-3, LNCaP, and LNCaP-C4-2B), and dorsolateral prostate tissues from control and SFN-treated TRAMP mice [8] to determine the role of Notch1, Notch2, and Notch4 in anticancer effects of SFN. The gene discussed is NOTCH4; the disease is prostate cancer.