In conclusion, the results of the present not only reveal in vitro and in vivo differences in effect of SFN on Notch activation but also indicate that activation of Notch1, Notch2, and Notch4 is largely dispensable for cellular responses to SFN (e.g., inhibition of cell migration or apoptosis) at least in human prostate cancer cells. This evidence concerns the gene NOTCH2 and Familial prostate cancer.