RAB5A and infection: The presence of wortmannin did not affect DENV-2 NGC penetration, as shown by the loss of capsid protein immunofluorescence after 60 min of infection similarly to control cells, but in cells infected with DENV-2 16681 the dotted fluorescence pattern of C protein still remained colocalizing with Rab5, confirming that the blockade in early endosome maturation prevented the cellular trafficking of this virus strain and its fusion.