Although methylation of KIAA1199 has been reported in normal colon mucosa and demethylation in human colon carcinoma specimens by a high-throughput analysis [6], there is no detailed analysis of the quantification of methylation at individual CG pairs for KIAA1199. Our study demonstrated for the first time mapping of specific methylated cytosines in the CpG island of KIAA1199. The gene discussed is CEMIP; the disease is colon carcinoma.