Therefore, flow cytometry using E-Ig chimera and carbohydrate antibodies was performed to test for E-selectin ligand activity and to compare carbohydrate expression profiles of several widely studied breast cancer cell lines: ZR-75-1, BT-20, MDA-MB-468, T-47D, MDA-MB-231, MCF-7, and Hs-578t. The gene discussed is SELE; the disease is breast carcinoma.