In the present study, we tested the hypothesis that Treg depleted Hi-expressor A-subunit transgenics primed with TSHR A-subunit protein plus adjuvant and boosted with A-subunit adenovirus would develop thyroiditis, thyroid damage and antibodies to Tg, as well as antibody spreading to another thyroid antigen, TPO. This evidence concerns the gene TPO and thyroid gland disorder.