In the present study, we tested the hypothesis that Treg depleted Hi-expressor A-subunit transgenics primed with TSHR A-subunit protein plus adjuvant and boosted with A-subunit adenovirus would develop thyroiditis, thyroid damage and antibodies to Tg, as well as antibody spreading to another thyroid antigen, TPO. The gene discussed is TPO; the disease is thyroiditis.