Previously we found that massive thyroiditis, associated with thyroid damage and thyroid antibody spreading to Tg and TPO, was induced by Treg depletion using anti-CD25 before immunization with TSHR adenovirus in transgenic mice expressing low, but not high, levels of the human TSHR A-subunit in the thymus and thyroid [2], [4]. This evidence concerns the gene TG and thyroid gland disorder.