As activation of NF-kappaB induces the transcription of a variety of NF-kappaB target genes, we performed semi-quantitative RT-PCR analysis to quantify the expression levels of selected NF-kappaB target genes, including CCND1BcL-XLTNF-αVEGF-C and MYC, in Bmi-1-overexpressing, -silenced and vector control-glioma cells. The gene discussed is BMI1; the disease is central nervous system cancer.