Conversely, osteoclast differentiation was prevented by CM from PC3 cells knockdown of RUNX2 (D) suggesting that RUNX2 regulates RANKL expression, and that secretion of RANKL by metastatic prostate cancer cells in the bone microenvironment may support osteoclastogenesis and osteolysis. This evidence concerns the gene TNFSF11 and prostate carcinoma.