The results of the present study clearly showed that gingival tissue PTX3 levels were not increased in experimental periodontitis model with 40-day period, contrary to the gingival crevicular fluid findings of Pradeep et al. which reported that concentration of PTX3 in gingival crevicular fluid is increased in proportionately with the severity of periodontal disease [8]. This evidence concerns the gene PTX3 and periodontitis.