Indeed, in mouse colon carcinogenesis we observed a significantly increased expression of CD133, assessed by immunohistochemistry, in early neoplastic lesions which tended to decrease with tumour development, although remaining always higher in cancer than in normal adjacent tissues[38] and an increased CD133 expression, assessed using a quantitative reverse-transcription PCR, was reported in Dukes A compared to Dukes B and C colon cancers[39]. Here, PROM1 is linked to malignant colon neoplasm.