To investigate the roles of EZH2, CTNNB1 and CDH1 in HCC, we knocked-down the expression of EZH2 and CTNNB1 with shRNA and over-expressed CDH1 with pcDNA3.1-CDH1 expression vector in SK-HEP-1 cells as described previously [16], [21], [22]. This evidence concerns the gene CTNNB1 and hepatocellular carcinoma.