The co-infection of HIV-1/HCV led to the down-regulation of cytoskeletal protein tropomyosin alpha-4 isoform 1 (spot 1), the glycolytic protein pyruvate kinase isozymes M1/M2 (spot 5), an ER ATPase (spot 7), the anti-apoptosis and apoptosis regulator enzyme GST (spot 8), EIF3K (spot 9–2.57-fold), the cell growth protein GTPase Ran (spot 10), the chaperone HSP90-beta (spot 11–2.77-fold), and the stress-induced phosphoprotein 1 (STIP-1) (spot 13–2.82-fold). The gene discussed is RAN; the disease is coinfection.