To determine whether activation of NADPH oxidase activity occurred during OGD incubation or during the subsequent reperfusion, as previously reported in cortical neuronal cells [7], we subjected 6-day retinoic acid differentiated SH-SY5Y neuroblastoma cells to 40-minutes of OGD followed by reperfusion of cultures for various incubation times in the presence of dihydroethidium (DHE) (Figure 1A). The gene discussed is FMO5; the disease is neuroblastoma.